摘要目的 探讨凋亡抑制基因生存素、bcl-2在皮肤鳞状细胞癌(简称鳞癌)皮损及皮肤鳞癌细胞系(SCL-1)细胞中的表达意义.方法 细胞免疫组化观察60例鳞癌患者皮损中生存素、bcl-2蛋白的表达并进行统计分析.Western印迹检测HaCaT细胞系和皮肤鳞癌细胞系(SCL-1)细胞中生存素bcl-2蛋白的表达.结果 免疫组化示正常皮肤组织中未见bcl-2与生存素表达;生存素、bcl-2在鳞癌皮损中呈阳性表达,bcl-2、生存素蛋白表达阳性率分别为70%、60%,两者表达无相关性(P>0.05).生存素的表达与年龄、性别、发病部位、病理分级无明显关系,与有无淋巴结转移密切相关.bcl-2蛋白的表达与年龄、性别、发病部位,淋巴结转移无明显关系,但随着鳞癌肿瘤病理分级的升高而降低.Western印迹分析示生存素、bcl-2蛋白在SCL-1细胞中表达明显高于HaCaT细胞.结论 生存素、bcl-2在鳞癌的发生发展中,可能通过不同的抗凋亡路径发挥作用.

abstractsObjective To investigate the expression of survivin and bcl-2 in human squamous cell carcinoma (SCC) lesions and cell line SCL-1. Methods Tissue samples from 60 patients with SCC and 10 normal human controls were immunohistochemically stained to detect the expressions of survivin and bcl-2.Western blot was used to measure the expressions of bcl-2 and survivin proteins in HaCaT human keratinocytes and SCL-1 human squamous cell carcinoma cells. Results In normal control tissues, there was no expressions of survivin or bcl-2, while in SCC, the expression rates of bcl-2 and survivin were 70% and 60%, respectively,and there was no statistical correlation between the expressions of bcl-2 and survivin (P >0.05). Neither the expression of survivin nor that of bcl-2 was correlated to patients' age, gender or lesional site (all P >0.05). A statistical correlation was observed between the pathological stage in patients and expression of bcl-2 as well as between lymph node metastasis and expression of survivin (both P < 0.05). Western blot analysis revealed a significant increase in the expression of survivin and bcl-2 in SCL-1 cells compared with HaCaT cells. Con-clusion In SCC, survivin and bcl-2 seem to play their roles via different anti-apoptotic pathways.