摘要目的 研究黄芪对核转录因子kappaB(nuclear factor of kappaB,NF-kappaB)p65和肌肉环状指蛋白1(muscle RING finger protein 1,MuRF1)活性的影响,探讨黄芪防治肌萎缩的作用及其机制.方法 54只Wistar大鼠,建立右下肢腓肠肌失神经支配模型,随机分为黄芪治疗组和对照组.采用逆转录聚合酶链反应(RT-PCR)和Western bloting检测术后2 d、7 d、14 d和28 d时大鼠腓肠肌NF-kappB p65和MuRF1的mRNA和蛋白表达水平,并结合肌肉湿重比分析其相关性.结果 大鼠腓肠肌失神经支配后NF-lcappaB p65和MuRF1的mRNA和蛋白表达在各时间点持续增加(P<0.05).术后7 d、14 d和28 d黄芪治疗组腓肠肌湿重比高于同期失神经对照组(P<0.05),而其NF-kappaB p65和MuRF1的表达则低于相应失神经对照组(P<0.05).结论 黄芪可以有效延缓去神经骨骼肌的萎缩,其作用机制与拮抗NF-kappaB p65、下调MuRF1的表达有关.

abstractsObjective To study the influence of Radix Astragali on the expression of nuclear factor of kappaB (NF-kappaB)p65 and muscle RING finger protein l(MuRF1)and explore the preventive and therapeutic effect and mechanisms of Radix Astragali on prevention of denervation muscle atrophy. Methods Denervation of the right gastrocnemius muscle was created in 54 Wister rats. The animals were randomized into Radix Astragali treatment group and control group. Levels of NF-kappaB p65 and MuRF1 mRNA and protein in the gastrocnemius muscle at 2, 7, 14 and 28 days after denervation were detected by RT-polymerase chain reaction (RT-PCR)and Western Blot. The ratio of muscle wet weight was also analyzed for comparison. Results After gastrocnemius muscle denervation, the levels of NF-kappaB p65 and MuRFl mRNA and protein increased with time( P < 0.05). On the 7th , 14th and 28th day after denervation muscle wet weight of the Radix Astragali treated group was heavier than that of the control group (P < 0.05), while expression of NF-kappaB and MuRFl was remarkably lower than that of the control group at the same time points( P < 0.05) . Conclusion Radix Astragali can significantly delay atrophy of denervated skeletal muscle. This effect may be related with its inhibition of the NF-kappaB/MuRFl pathway.