摘要新冠病毒等高致病性病原体必须在生物安全三级(BSL-3)实验室进行研究，并且毒株获取困难，给科研和防治工作的开展带来了限制。假病毒是一种嵌合型病毒颗粒，结构与野生病毒相似，具有类似活病毒的生物特性，但没有活病毒的致病性，可以在BSL-2实验室进行研究，并且可通过插入报告基因，实现假病毒的定量和定性检测。假病毒技术已经被广泛应用于中和抗体检测和疫苗免疫效果评价、病毒受体研究、动物模型建立、抗病毒药物筛选和核酸诊断试剂的质控等研究。为了更好的开展新型冠状病毒等新发、突发、烈性传染病病原体中和抗体检测、疫苗的研发与评价和药物筛选等研究，本文就假病毒技术研究进展和应用进行综述。

abstractsSevere acute respiratory syndrome coronavirus 2 and other highly pathogenic pathogens need to be studied in the Biosafety Level 3 (BSL-3) laboratory, and it is difficult to obtain live viruses, which limits the development of scientific research and prevention. Pseudovirus is a recombinant virus with similar structure and biological characteristics to wild-type virus without the pathogenicity of live viruses. Pseudovirus can be studied in the BSL-2 laboratory, and it can be detected quantitatively and qualitatively by carried reporter gene. Pseudovirus has been widely used for detection of neutralizing antibody and evaluation of vaccines, receptor recognition, establishment of animal models, antiviral drug screening and quality control of nucleic acid detection etc. In order to better carry out detection of neutralizing antibody, evaluation of vaccine and drug screening etc. of SARS-CoV-2 and other new, emerging and severe infectious disease pathogens, this article reviews the research progress and application of pseudovirus technology.