摘要目的:探究环状RNA hsa_circ_0007291(circSCAP)对肺腺癌恶性生物学行为的影响。方法:从数据库获取circSCAP的线性序列，通过在线性序列两端加入Alu元件的方式构建circSCAP的过表达质粒，转染过表达circSCAP的质粒于A549细胞中，通过克隆形成实验与小室实验评估过表达circSCAP对肺腺癌细胞功能的影响。通过MS2 RNA下拉实验与质谱技术结合，寻找能够与circSCAP结合的蛋白，并对这些蛋白进行通路富集分析。采用 t检验分析两组差异。 结果:构建质粒可成功上调circSCAP的表达。克隆形成实验结果表明，与对照组比较[(186.7±15.3)克隆/孔]，过表达circSCAP可提高A549细胞的克隆形成能力[(771.0±46.2)克隆/孔， t＝20.810， P<0.01]；而小室实验结果也提示，与对照组比较[(45.0±7.5)个/视野]，过表达circSCAP可提高A549细胞的侵袭能力[(73.0±6.0)个/视野， t＝5.000， P<0.01]。通过MS2 RNA下拉实验与质谱检测，可见17个可与circSCAP结合的蛋白，通路分析发现这些蛋白显著富集在氨基酸生物合成与细胞外基质受体相互作用通路中。 结论:过表达环状RNA circSCAP可以促进肺腺癌细胞的增殖与侵袭能力，circSCAP可能与蛋白结合发挥生物学功能。

abstractsObjective:To investigate whether circular RNA has_circ_0007291 (circular RNA SCAP, circSCAP) can influence the malignant behavior of lung adenocarcinoma.Methods:A plasmid was constructed to overexpress circSCAP by flanking Alu elements besides two ends of linear circSCAP sequence obtained from public database. circSCAP was overexpressed in A549 cells by transfecting plasmids, and colony formation assay and Transwell assay were performed to evaluate proliferation and invasion ability of A549 cells. MS2 RNA pull-down assay followed by mass spectrometry was performed to identify proteins binding with circSCAP. Student’s t test was performed and P<0.05 was considered as statistically significant. Results:circSCAP was successfully overexpressed in A549 cells. Overexpression of circSCAP [(771.0±46.2) colonies/well, t=20.81, P<0.01] significantly increased proliferation ability of A549 cells as compared with control group [(186.7±15.3) colonies/well, t=20.810, P<0.01] as revealed by colony formation assay. Transwell assay also confirmed that circSCAP overexpression [(73.0±6.0) cells/view] promoted invasion of A549 cells as compared with control group [(45.0±7.5) cells/view, t=5.000, P<0.01]. MS2 pull-down assay identified 17 proteins binding with circSCAP. Pathway enrichment analyses showed these proteins were enriched in pathways of biosynthesis of amino acids and ECM-receptor interaction. Conclusion:Overexpression of circSCAP promoted proliferation and invasion ability of A549 cells, and circSCAP may bind with proteins to exert its function.