摘要目的 寻找肿瘤性胆囊息肉样病变(PLG)患者血清中的特异性蛋白,并建立诊断模型.方法 应用表面增强激光解吸电离飞行时间质谱技术(SELDI-TOF-MS)和WCX型磁珠检测23例肿瘤性PLG、21例非肿瘤性PLG和26例正常人的血清蛋白质指纹图谱,并联合应用Biomarker wizard及Biomarker Patterns Software软件分析处理数据.结果 初步筛选出对诊断肿瘤性PLG有代表性的22个特异性蛋白,分析系统在设定条件下筛选出3个特异性蛋白(5064.52 Da,7916.33 Da,7643.32 Da)用于建立肿瘤性PLG的诊断模型.此模型对肿瘤性胆囊息肉样病变诊断的敏感度为100%,特异度为89.4%.结论 SELDI-TOF-MS技术可筛选出肿瘤性PLG的特异性蛋白并建立起肿瘤性PLG的诊断模型.

abstractsObjective To detect the serum specific proteins in tumor-like polypoid lesions of the gallbladder patients and establish diagnostic model.Methods Surface enhanced laser desorption/ionization time of flight mass spectrometry(SELDI-TOF-MS)technique and WCX Magnetic Beads were used to detect the serum Droteomic patterns of 23 patients with tumor-like PLG,21 patients with non tumor-like PLG and 26 normal persons.Biomarker Wizard and Biomarker Patterns Software were used in combination to analyze the data.Resuits Preliminary screening out 22 representative specific proteins for the diagnosis of the tumor like PLG.Analysis system under the conditions set selected 3 specific proteins to establish diagnostic model for the tumor-like PLG.The sensitivity and specificity of the model for the diagnosis of the tumor-like PLG were 100%and 89.4%,respectively.Conclusion SELDI-TOF-MS technique can select specific protein of the tumor-like PLG,and establish diagnostic model of the tumor-like PLG.