摘要目的 探讨抑郁障碍对非M3型急性髓系白血病(AML)患者调节性T细胞(Treg细胞)的影响.方法 63例初诊非M3型AML患者纳入研究,健康对照为25名健康体检者.于化疗前对患者的抑郁程度进行评估,并于每个疗程化疗后骨髓恢复期采用流式细胞术对外周血单个核细胞的Treg细胞量进行检测.结果 所有疗程结束后,AML患者Treg细胞比例为(6.48±1.81)％,明显高于对照组的(4.99±1.29)％(P=0.001),但第1疗程不同程度抑郁组患者Treg细胞比例差异无统计学意义,第2、3疗程后,Treg细胞比例随抑郁程度的增高而呈同步增高的趋势.控制年龄因素的偏相关分析表明:HAMD积分与第1疗程后的Treg细胞比例无相关性(相关系数为0.120,P=0.345);HAMD积分与第2、3疗程后的Treg细胞比例呈正相关(相关系数分别为0.619、0.834,P值均＜0.05).结论 抑郁程度可影响AML患者骨髓单个核细胞的Treg细胞比例,且在至少化疗2个疗程后方显现.

abstractsObjective To investigate the influence of depression levels on regulatory T cells (Tregs) in patients with non-M3 acute myeloid leukemia (AML).Methods A total of 63 patients with primarily diagnostic non-M3 AML and 25 healthy controls were enrolled,and the levels of depression by using HADM score and the percentages of Tregs by flow cytometry were evaluated in pre-treatment and post-treatment,respectively.Results After every course of chemotherapy,the percentages of Tregs of PBMNC in AML showed the higher level of (6.48±1.81)％ than those of (4.99± 1.29)％ in control (P=0.001).There was no difference among different levels of depression groups after the first cycle.However,the percentages of Tregs increased with the worse of depression after the second and third cycles.Partial correlation analysis after adjusting age indicated that the percentages of Tregs showed no correlation with the levels of depression after the first cycle (correlation coefficient,0.120,P=0.345),and showed the positive correlation with depression levels after the second and third cycles (correlation coefficient,0.619 and 0.614,all P values ＜0.05).Conclusion The depression levels showed an association with the percentages of Tregs in patients with non-M3 AML,which could be observed only after the second cycles of chemotherapy.