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目的 观察人视网膜色素上皮(RPE)细胞人类白细胞抗原(HLA)-DP、-DQ、-DR抗原和CD40抗原的表达,以确定它们在免疫应答过程中的分子表达和刺激T淋巴细胞活化的能力.方法 人RPE细胞分别在γ-干扰素诱导和无诱导的条件下培养,免疫组织化学染色方法测定HLA-DP、-DQ、-DR抗原和CD40抗原在RPE细胞中的表达.体外共同培养人RPE细胞和外周血单个核细胞,荧光活化细胞分类仪测定其中活化的T淋巴细胞CD69的表达.结果 γ-干扰素能够诱导HLA-DP、-DQ、-DR抗原表达升高和CD40在人RPE细胞上的表达,CD69阳性的T淋巴细胞,在人RPE细胞与外周血单个核细胞的共同培养系统中的含量增加.结论 人RPE细胞能够激活外周血中T淋巴细胞,是一种具有免疫学特性潜能的抗原递呈细胞.

Objective To investigate the expression of Human leucocyte antigen(HLA)-DP,-DQ,-DR and CD40 in human retinal pigment epithelial(RPE)cells,to determine their molecule expression in immune response process,and their abilities to stimulate T lymphocyte activation. Methods Human RPE cells were cultured with or without(-IFN respectively.Expression of HLA-DP,-DQ,-DR and CD40 was measured by immunohistochemical staining.Meanwhile,peripheral blood mononuelear cells(PBMC)were co-cultured with RPE cells in vitro,and then the expression of activated lymphocytes CD69 was measured by fluorescence activated eell sorter(FACS). Results Expression of HLA-DP,-DQ,-DR and CD40 antigen were enhanced by γ-interferon inducement.Increasing amount of CD69 positive Iymphocytes were found in the co-culture system of RPE cells and PBMC. Conclusion T-lymphocytes in the peripheral blood were activated by human RPE cells which is antigen presenting cells with immunological characteristics potential.