摘要[目的]观察持续频闪光刺激对发育敏感期豚鼠视网膜组织及功能的影响.[方法]24只出生日龄14 d的豚鼠随机分为频闪组和对照组,每组均为12只.频闪组使用频闪调光器以0.5 Hz频率等时交替频闪,照度波动0～500 Lux；对照组给予500 Lux照明.2组均使用500 nm波长发光二极管,控制光照时间为昼夜12h轮替.实验第12周所有豚鼠行跟底彩色照相和闪光视网膜电图(F-ERG)检查.检查结束后摘除眼球,测量眼球水平径、垂直径及前后径3条径线,光学显微镜和透射电子显微镜观察眼球后极部结构改变.[结果]与对照组比较,频闪组豚鼠眼底漆裂纹样改变更为明显,ERG a波潜伏期延长;眼球水平径、垂直径及前后径分别较对照组增加(0.89±0.30)、(0.69±0.20)、(0.96±0.30)mm,差异有统计学意义(t=12.7、11.9、15.8,P＜0.05).光学显微镜观察发现,频闪组豚鼠巩膜纤维出现扩张；透射电子镜显微镜观察发现,视网膜感光细胞层外段排列稀疏紊乱并可见大量脱落外节盘膜.[结论]持续频闪光刺激会诱导豚鼠眼球产生过度发育,并会影响视网膜结构与传导功能.

abstracts[Objective] To observe the effect of persistent flickering stimulus on the structure and function of retina in guinea pigs during a developmentally sensitive period.[Methods] Twenty-four twoweek-old guinea pigs were randomly divided into flicker light (FL) group and control group,with 12 guinea pigs in each group.Animals in FL group were raised under 500 Lux illumination with a duty diurnal cycle of 50％ at a flash rate of 0.5 Hz.Animals in control group were reared under steady 500 Lux illumination.Light emitting diode (LED) lamps were used for lighting under a 12-hour light/12-hour dark cycle.After the collection of fundus photographs and electroretinograms recorded at week 12,eyeballs were taken out,three dimensions were measured,and histopathological changes were examined.[Results] Compared to control group,tessellated fundus in FL group appeared more prevalent; implicit time of “a” waves were prolonged in electroretinogram; the eyeballs were increased in horizontal,vertical,axial dimensions by (0.89±0.30),(0.69±0.20) and (0.96±0.30) mm respectively,the differences between two groups were statistically significant (t =12.7,11.9,15.8; P ＜ 0.05 ).The gap of sclera collagen fiber was slightly widened.The photoreceptor layer was more likely to develop a disordered outer segment,which contained deciduous disc membranes.[Conclusion] Persistent flickering stimulus is attended by development of excessive ocular enlargement,which could affect the retinal structure and function of photoreceptors.