摘要目的 探讨高分辨率光相干断层扫描(Cirrus HD-OCT)测量正常眼黄斑区节细胞-内丛状层(GCIPL)厚度的一致性与可重复性.方法 54名正常者108只眼纳入研究.其中,男性26名52只眼,女性28名56只眼.年龄19～75岁,平均年龄(46.2±16.5)岁.两名检查者采用Cirrus HD-OCT黄斑容积512×128扫描程序对受检者双眼黄斑区扫描3次;再由一名检查者以黄斑容积200×200扫描程序对受检者右眼黄斑区扫描3次.测量受检眼平均、最小、颞上方、上方、鼻上方、鼻下方、下方及颞下方GCIPL厚度.计算同一检查者内和不同检查者间、同一扫描程序内与不同扫描程序间的类内相关系数(ICC)值,分析测量的一致性.随机选取10名受检者,由一名检查者采用黄斑容积512×128扫描程序对其左眼黄斑区连续扫描10次,计算其GCIPL厚度值变异系数(CV),评估其重复性与测量精度.结果 两名检查者以黄斑容积512×128扫描程序测得受检者双眼黄斑区GCIPL厚度的ICC值分别为0.959～0.995、0.955～0.997;两名检查者之间相应的ICC值为0.944～0.993.同一检查内及不同检查者之间,其ICC值均＞0.800;ICC值最高者为平均GCIPL厚度,ICC值最低者为最小GCIPL厚度.一名检查者分别以黄斑容积512×128、200×200扫描程序测得受检者右眼黄斑区GCIPL厚度的ICC值分别为0.984～0.996、0.927～0.997;两种扫描程序之间相应的ICC值为0.966～0.994.同一扫描程序及不同扫描程序之间,其ICC值均＞0.800.一名检查者以黄斑容积512×128扫描程序测得10只受试眼黄斑区GCIPL厚度值的CV为(0.70±0.31)％～(1.35±0.86)％.结论 Cirrus HD-OCT测量正常眼黄斑区GCIPL厚度的一致性与可重复性好.

abstractsObjective To evaluate the repeatability and reproducibility of macular ganglion cell-inner plexiform layer (GCIPL) thickness measurement using spectral-domain optical coherence tomography (Cirrus HD-OCT).Method One hundred and eight eyes of 54 normal subjects (26 males and 28 females) between 19 and 75 years of age [mean age (46.2± 16.5) years] were included.Each eye underwent macular scanning using Cirrus HD-OCT Macular Cube 512 × 128 protocol by two operators.Three scans of each eye were obtained by each operator.For the right eye of each subject,three extra scans were obtained using Macular Cube 200 × 200 protocol by one operator.The average,minimum,superotemporal,superior,superonasal,inferonasal,inferior,and inferotemporal GCIPL thickness was analyzed and the repeatability of GCIPL thickness measurement was evaluated with intra-operator,inter-operator,intra-protocol,and inter-protocol intraclass correlation coefficients (ICC).Ten extra scans were obtained from the left eyes of 10 randomly selected subjects for reproducibility assessment with coefficients of variation (CV).Results The intra-operator ICC of macular GCIPL measurement using Macular Cube 512 × 128 protocol by two operators were 0.959-0.995 and 0.954-0.997,respectively; and the inter-operator ICC were 0.944-0.993.All intra-and inter operator ICC were ＞0.800 with the highest and lowest records of the average and minimum GCIPL thickness,respectively.The intra-protocol ICC of Macular Cube 512 × 128 protocol and Macular Cube 200× 200 protocol were 0.986-0.996 and 0.927-0.997,respectively; and the inter-protocol ICC were 0.966 0.994.All intra-and inter-protocol ICC were ＞0.800.CV of GCIPL thickness measurement using MacularCube 512×128 protocol were (0.70±0.31)％ (1.35±0.86)％.Conclusion Cirrus HDOCT can measure macular GCIPL thickness in normal eyes with excellent repeatability and reproducibility.