摘要目的 观察芬戈莫德(FTY720)对视网膜光损伤大鼠光感受器细胞及小胶质细胞的影响.方法 Sprague-Dawley大鼠120只随机分为正常组、模型组、溶媒组和FTY720组,每组30只.正常组大鼠不予处理;模型组、溶媒组和FTY720组大鼠建立光损伤动物模型.模型组大鼠仅接受光照.FTY720组大鼠建模前腹腔注射FTY720,溶媒组大鼠注射50％二甲基亚砜.光照后6h及1、3、7d,采用流式细胞仪检测大鼠小胶质细胞所占比例;酶联免疫吸附试验检测大鼠视网膜中白细胞介素(IL)-1β的含量.光照后1d,采用原位末端标记法检测光感受器细胞凋亡情况.光照后7d,采用苏木精-伊红染色观察大鼠视网膜形态结构.结果 大鼠视网膜中小胶质细胞所占比例及IL-1β含量均于光照后6h开始升高,光照后3d达高峰,光照后7d开始下降.光照后6h及1、3、7d,FTY720组大鼠视网膜中小胶质细胞所占比例及IL-1β含量较正常组升高,较模型组、溶酶组明显降低,差异均有统计学意义(P＜0.05).光照后1d,正常组、模型组、溶酶组及FTY720组大鼠视网膜凋亡细胞比例分别为0、(87.66±2.50)％、(86.00±2.44)％、(49.66±2.80)％.FTY720组大鼠视网膜凋亡细胞比例较正常组升高,较模型组、溶酶组明显降低,差异均有统计学意义(P＜0.05).光照后7d,正常组大鼠视网膜结构清晰,细胞排列整齐;溶酶组、模型组大鼠视网膜结构不清楚,细胞排列紊乱,外核层明显变薄;FTY720组大鼠视网膜结构清晰,外核层厚度较溶酶组、模型组厚,但薄于正常组.结论 FTY720能抑制视网膜光损伤大鼠光感受器细胞的凋亡及小胶质细胞的活化.

abstractsObjective To investigate the effects of FTY720 on retinal photoreceptor cells and microglial following light-induced degeneration in rat retina.Methods 120 Sprague-Dawley rats were randomly divided into four groups including FTY720 group,solvent control group,model group and normal group.The rats of normal group were not intervened.The FTY720 group,solvent control group and model group establish retinal light injury mode.FTY720 was injected into abdominal cavity of the rats in FTY720 group 0.5 hours before light exposure.50％ dimethylsulfoxide was injected into abdominal cavity of the rats in solvent control group.The expressions of microglial cells in rat retinal were quantified using flow cytometry,the expressions of interleukin (IL)-1β were examined by enzyme-linked immuno sorbent assay at 6 hours,1 day,3 days,7 days after light exposure.The apoptosis of retinal photoreceptor cells were measured by terminal-deoxynucleoitidyl transferase mediated nick end labeling at 1 day after light exposure.The morphological change of retinal were viewed by haematoxylin and eosin staining at 7 days after light exposure.Results The expressions of microgilal and IL-1β began to rise at 1 day after light exposure,reached at peak at 3 days and decreased at 7 days.The expressions of IL-1βand microglial in FTY720 group were significantly lower than solvent control group and model group,but higher than normal group (P＜0.05).One day after exposure to light,the apoptosis cell ratio in normal group,model group,solvent control group and FTY720 group were 0,(87.66 ± 2.50) ％,(86.00 ± 2.44) ％,(49.66 ± 2.80) ％.The apoptosis cell in FTY720 group were higher than normal group,lower than solvent control group and model group (P＜0.05).Seven days after exposure to light,the retinal in normal group was structured and the cell was arranged well,the cell in solvent control group and model group was irregular arrangement and the outer nuclear layer (ONL) was thin after light exposure.The thickness of the ONL in FTY720 group was significantly higher than solvent control group and model group,below normal group.Conclusion FTY720 can prevents retinal photoreceptor cells from apoptosis and inhibits activation of microglial.