摘要目的 利用基因表达芯片技术和连接网络(CMAP)技术筛选干预缺氧诱导视网膜血管内皮细胞模型的小分子化合物和药物.方法 将前期研究获取的CoCl2诱导的缺氧人胚视网膜微血管内皮细胞模型中包括上调和下调的326个差异表达基因建立成查询签名格式文件,进入CMAP.通过疾病特征性差异基因谱与CMAP中的对照基因表达谱之间的逐一比对,获取正相关和负相关的小分子化合物和药物.进一步检索文献,了解其作用机制,对照早产儿视网膜病变(ROP)的发病机制,筛选与ROP正相关或负相关的化合物.结果 差异表达基因CMAP检索发现,44种小分子化合物和药物与ROP存在正相关;18种小分子化合物和药物与ROP存在负相关.文献检索发现,环吡酮胺、CoCl2、棉酚和醉茄素A与ROP有正相关;环腺苷酸、去甲骆驼蓬碱、柚皮甙和丙磺舒与ROP有负相关.结论 环吡酮胺、CoCl2、棉酚和醉茄素A与ROP有正相关;环腺苷酸、去甲骆驼蓬碱、柚皮甙和丙磺舒与ROP有负相关.

abstractsObjective To screen compounds or drugs can affect the hypoxia induced-gene expression of retinal vascular endothelial cell based on gene expression microarrays and connectivity map (CMAP)technology.Methods Totally 326 up-regulated and down-regulated genes of hypoxic human embryonic retinal microvascular endothelial cells minduced by cobalt chloride in the previous study were converted into query signature format documents.Gene profile of the disease characteristics was then compared with that of control in CMAP website database,positive and negative compounds related to retinopathy of prematurity (ROP) were finally screened out.Results 44 and 18 compounds or drugs have positive and negative relationship with ROP respectively by searching CMAP database with differentially expressed genes.Ciclopirox,cobalt chloride,gossypol and withaferin A have positive relationship with ROP.Cyclic adenosine monophosphate,harmalol,naringin and probenecid have a negative effect on ROP.Conclusions Ciclopirox,cobalt chloride,gossypol and withaferin A have a positive effect on ROP.However,cyclic adenosine monophosphate,harmalol,naringin and probenecid have a negative effect.