摘要目的　建立一种简便可靠的筛选抗肿瘤细胞生长和诱导凋亡药物的方法。方法　以细胞浆中酸性磷酸酶活力和细胞增殖之间具有紧密关系为依据，确定细胞数目与酸性磷酸酶活力之间的相关性，建立96孔板微量测定方法，同时运用流式细胞仪检测细胞周期和凋亡，确定凋亡细胞与酸性磷酸酶的关系，并与噻唑兰(MTT)方法进行灵敏度的比较。结果　在2种肝癌细胞株（Hep G2细胞和CBRH-7919细胞）中，酸性磷酸酶的活力与细胞数目呈正比关系，在(0.5～7）×103细胞数范围内呈直线关系，二者相关系数（CV）达0.994；在佛波酯（TPA）刺激肝癌细胞1 h后，酸性磷酸酶的活力即显著上升。相反在三氧化二砷作用后，酸性磷酸酶的活力即显著下降，浓度越高，下降越显著。三氧化二砷作用Hep G2细胞24 h，凋亡细胞仅占3.98%，作用CBRH-7919细胞，在还没有出现凋亡时，酸性磷酸酶的活力即已显著下降。结论　检测细胞的酸性磷酸酶活力可作为细胞增殖和凋亡的指标，此方法简便、快速，可用于大批量抗肿瘤药物的筛选。

abstractsObjective　To establish a simple assay for quick scanning of effective agents in growth inhibition and apoptosis induction. Methods　After observing the correlation between cell number and acid phosphatase activity, and between cell apoptosis and acid phosphatase activity, we established a microplate densimetry method to measure the acid phosphatase activity. We also compared the sensitivity with MTT assay and confirmed cell apoptosis by flow cytometry. Results　In two cell lines of cancer (Hep G2 and CBRH-7919 cells), the acid phosphatase activity was correlated well with the cell number, and was directly proportional to the cell number in the range of 0.5×103～0.7×103. The coefficient reached to 0.994. After stimulation of phorbel ester (TPA) for one hour, the acid phosphatase activity rose significantly. In contrast, after the inhibition of cell growth by As2O3, the acid phosphatase activity decreased significantly. The higher the concentration of As2O3, the lower the acid phophatase activity. The acid phophatase activity went down significantly when apoptosis in Hep G2 cells was only 3.98% and no apoptosis in CBRH-7919 cells after 24-hour treatment with As2O3. Conclusions　The acid phophatase activity of cell may be used to measure cell proliferation and apoptosis. Because of the easiness and quickness, acid phophatase assay might be an ideal approach to scan the effective growth inhibitors and apoptosis inducers in a large number of drugs.