摘要目的 探讨2型糖尿病患者血小板膜糖蛋白Ⅵ(Glycoprotein Ⅵ,GP Ⅵ)的变化及其临床意义.方法 应用流式细胞术观察56例2型糖尿病患者和61名正常对照者血小板膜GPⅥ的表达水平,采用ELISA方法检测30例糖尿病患者血浆中可溶性GP Ⅵ分子表达水平,同时检测糖尿病患者P-选择素(CD62P)阳性率和糖化血红蛋白(HbA1c)水平.结果 糖尿病组血小板膜GPⅥ的平均几何荧光强度为93.66±35.24,正常对照组为62.83±24.20,糖尿病组GPⅥ表达水平明显高于正常对照组(t=-4.927,P＜0.05).30例糖尿病患者中,有9例血浆中可溶性GPⅥ为阳性,阳性率为30%,且与血小板膜GPⅥ含量呈负相关(r=-0.633,P＜0.05),而与CD62P及HbA1c无相关性,r值分别为:-0.333、-0.417,P均＞0.05.糖尿病患者血小板膜GPⅥ含量与CD62P、HbA1c无相关性,r值分别为:-0.009、-0.217,P均＞0.05.结论 糖尿病患者血小板膜GPⅥ表达增高,检测血小板膜表面GPⅥ水平和血浆中可溶性GPⅥ水平对于预测糖尿病患者血栓形成的风险性以及了解糖尿病患者血小板活化状态有重要价值.

abstractsObjeetive To explore change of platelet glycoproteins Ⅵ(GPⅥ)in patients with type 2 diabetes mellitus and its clinical significance.Methods The surface expression of platelet glycoprotein Ⅵ was determined by flow cytometry in 56 patients with type 2 diabetes mellitus and 61 normal individuals.Plasma GP Ⅵ concentration was measured by ELISA in 30 patients with type 2 diabetes mellitus.Platelet surface CD62P was analyzed by flow cytometry and HbA1c was determined in Datients with type 2 diabetes mellitus.Results The geometric mean fluorescence of platelet surface GPⅥ in Datients with type 2 diabetes mellitus was 93.66±35.24,which was significantly enhanced compared with normal subjects (62.83±24.2)(t=-4.927,P＜0.05).Nine patients were positive in plasma GPⅥ among 30 Datients with type 2 diabetes mellitus.Plasma GP Ⅵ concentrations in 9 positive patients were conversely correlated with platelet surface GPⅥ expression(r=-0.633,P＜0.05).However,there was no correlation between platelet Surface CD62P and plasma HbA1c concentrations, and the correlation coemcient is -0.333 and -0.417,respectively(P＞0.05).There was no correlation between platelet surface GP Ⅵ expression with C062P and HbA1c in patients with type 2 diabetes mellitus and the conrrelation coefficient is -0.009 and -0.217,respectively(P＞0.05).Conclusions GPⅥ expression on platelet surface is elevated in Datients with type 2 diabetes mellitus and the determination of platelet surface GPⅥ and plasma GPⅥ concennlation may helP to prognosticate the risk of thrombotic events and may play an important role in evaluating platelet activation in patients with type 2 diabetes mellitus.