摘要目的:探讨紫球藻多糖提取物祛除毛细血管扩张的机制。方法:2023年1月10日至4月18日，于云南特色植物提取实验室，通过体外微血管内皮细胞的管腔形成实验和划痕实验探索不同浓度的紫球藻多糖提取物——紫球藻Pro对血管管腔形成和迁移能力的影响，采用体内鸡胚绒毛尿囊膜血管实验直观检测紫球藻Pro对血管收缩能力的影响，采用蛋白免疫印迹实验验证紫球藻Pro对微血管内皮细胞内皮素-1蛋白表达水平的促进作用。结果:当紫球藻Pro的处理浓度低于1.0%时，对微血管内皮细胞管腔形成的初期、成熟期和坍缩阶段均无影响，对血管迁移能力也无作用(均 P>0.05)；当紫球藻Pro的处理浓度达到3.0%时，对微血管内皮细胞管腔坍缩阶段有促进作用。处理24 h时，相比于空白对照组，部分管腔出现明显坍缩，但差异无统计学意义( P>0.05)；处理48 h后，所有形成的管腔均坍缩，反映在降低的单位视野管腔数( t＝9.02, P<0.05)和管腔总长度( t＝10.89, P<0.05)。划痕实验显示，当处理浓度达到3.0%时，微血管内皮细胞的迁移能力虽然不受影响( P>0.05)，但细胞会更早出现脱壁、萎缩的现象。3.0%浓度紫球藻Pro处理30 min后能够引起鸡胚绒毛尿囊膜血管的明显收缩；体外微血管内皮细胞在0.3%紫球藻Pro处理24 h后，内皮素-1蛋白表达水平有增加的趋势。 结论:紫球藻Pro祛除毛细血管扩张的作用可能在于促进了血管内皮细胞高表达内皮素-1，内皮素-1能够促进血管收缩，同时紫球藻Pro不影响血管的新生过程。

abstractsObjective:To investigate the mechanism of Porphyridium cruentum polysaccharides extract in reducing telangiectasis.Methods:Experiments were conducted between January 10, 2023, and April 18, 2023, at the Yunnan Characteristic Plant Extraction Laboratory. In vitro experiments were conducted to investigate the effect of different concentrations of Porphyridium cruentum polysaccharides extract (Porphyridium cruentum Pro) on tube formation and migration of microvascular endothelial cells, using tube formation assay and migration assay. In an in vivo experiment, a chick embryo chorioallantoic membrane assay was conducted to assess the impact of Porphyridium cruentum Pro on vasoconstriction. Finally, a western blot analysis was conducted to confirm the impact of Porphyridium cruentum Pro on increasing the protein level of endothelin-1 in microvascular endothelial cells.Results:When the concentration of Porphyridium cruentum Pro was below 1.0%, it did not significantly affect all the stages of tube formation and the migration ability of microvascular endothelial cells ( P>0.05). However, at a concentration of 3.0%, there was a noticeable effect on the collapse of the formed tubes. In comparison to the control group, some tubes started collapsing 24 hours post-treatment, despite the lack of statistical significance in the analysis. After 48 hours of treatment, all the tubes collapsed, as evidenced by a significant decrease in the number of tubes ( t=9.02, P<0.05) and total tube length ( t=10.89, P<0.05) per field. The migration assay study revealed that at a concentration of 3%, there was observed cell detachment and atrophy, while the cell migration capacity of microvascular endothelial cells remained relatively unchanged. When treated with a 3.0% concentration of Porphyridium cruentum Pro for 30 minutes, a noteble vasoconstriction was observed in the blood vessels of the chick embryo chorioallantoic membrane. Moreover, after a 24-hour treatment with 0.3% Porphyridium cruentum Pro, there was an elevation in endothelin-1 protein expression level in microvascular endothelial cells. Conclusions:The observed function of Porphyridium cruentum Pro in reducing telangiectasis may associated to its ability to enhance the expression of endothelin-1 in vascular endothelial cells, which in turn promotes the vasoconstriction. Meanwhile, the angiogenesis is not significantly affected by Porphyridium cruentum Pro.