摘要目的 探讨凋亡调控因子Survivin、半胱氨酸天门冬氨酸蛋白酶-3(caspase-3)在卵巢上皮性肿瘤中表达的临床意义.方法 采用免疫组织化学技术,检测2002年1月至2012年1月濮阳市第三人民医院收治的40例卵巢上皮性癌、10例交界性卵巢上皮性肿瘤组织及10例卵巢良性上皮性肿瘤中Survivin、caspase-3蛋白表达,并结合临床病理特征进行分析.结果 Survivin、caspase-3在卵巢上皮性肿瘤中表达呈负相关；Survivin在卵巢上皮性癌中表达率为72.5％ (29/40),且表达水平高于卵巢良性上皮性肿瘤(0)和交界性卵巢上皮性肿瘤(40.0％),P＜0.05.caspase-3在卵巢上皮性癌中表达率为0.5％ (2/40),且表达水平远远低于卵巢良性上皮性肿瘤和交界性卵巢上皮性肿瘤(P＜0.01).结论 Survivin可能抑制caspase-3在卵巢癌中表达,导致卵巢癌细胞增殖,凋亡受阻、肿瘤形成；Survivin基因的表达可能发生在卵巢癌恶性转化的早期；Survivin的表达与瘤细胞分化、增殖密切相关,并且其高表达可能会作为预后不良的标志；caspase-3在卵巢癌中极低表达,有望成为卵巢癌良恶鉴别新的指标.

abstractsObjective To investigate the expression of apoptosis regulatory factor Survivin and caspase-3 in epithelial ovarian tumor and its clinical significance.Methods The expression of proteins Survivin and caspase-3 in 40 cases of epithelial ovarian tumor,10 cases of borderline epithelial ovarian tumora and 10 cases of benign ethelial tumor were detected by immunohistochemistry; then the clinical and pathological features were analyzed.Results There was a negative correlation of Survivin and caspase-3 expression in epithelial ovarian tumor; the expression rate of Survivin in epithelial ovarian cancer was 72.5％ (29/40),and it was higher than that in epithelial ovarian tumor and borderline epithelial ovarian tumor(P ＜ 0.05).The expression rate of caspase-3 in epithelial ovarian cancer was 0.5％(2/40),and it was more lower than that in epithelial ovarian tumor and borderline epithelial ovarian tumor(P ＜0.01).Conclusions The expression of caspase-3 is inhibited by Survivin,which results in the ovarian cancer cells multiplication,apoptosis obstruction and tumor generation; The expression of Survivin gene happens in the early stage of ovarian cancer malignant transformation; The expression of Survivin is closely correlated with tumor cell differentiation and proliferation,and its high expression can be the identification marker of unfavourable prognosis; The expression level of caspase-3 in ovarian cancer is quite low,which makes it a new index to identify the benign and malignant ovarian tumor; The qualitative and quantitative analysis of Survivin,caspase-3 and PCNA may become the index of curative effect of drugs.