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摘要In the present study,we identify three type Ⅰ interferon(IFN)genes(Ad/AsIFNe1-3)and a type Ⅱ IFN gene(Ad/AsIFNγ)from the Dabry's sturgeon(Acipenser dabryanus)and the Chinese sturgeon(Acipenser sinensis).Sequence analysis revealed that Ad/AsIFNe1-3 and Ad/AsIFNγ contain several conserved characteristics,including signal peptides,interferon alpha,beta,and delta(IFabd)domains,and N-glycosylation sites.Ad/AsIFNe1-3 belongs to the type Ⅰ IFN group Ⅰsubgroup,possessing two conserved cysteines residues(C1 andC3),and Ad/As IFNγcontained a conserved nuclear localization sequence(NLS)motif.Ad/AsIFNe1-3 and Ad/AsIFNγ contain signature motifs indicative of their corresponding IFN group.The Ad/AsIFNe1-3 and Ad/AsIFNγ genes were found to consist of 5 exons/4 introns and 4 exons/3 introns,respectively.These IFNs were separated by four phase 0 introns(type ⅠIFN)and three phase 0 introns(type Ⅱ IFN).The sequences of IFNe1-3 and IFNγ from the Dabry's sturgeon and the Chinese sturgeon were closely aligned,suggested that these two species are closely related.Phylogenetic analysis revealed that Ad/AsIFNe1-3 and Ad/AsIFNγ clustered together with the corresponding homologous proteins from other fish species.AdIFNe1-3 were found to be high expressed in early embryonic development,suggesting that AdIFNe1-3 might indicate maternal transmission,while AdIFNγ may not mediate embryonic development.Tissue distribution analysis revealed that AdIFNe1-3 and AdIFNγ carry out biological functions in immune and non-immune tissues compartments.AdIFNe1-3 and AdIFNγ can be stimulated by polyinosinic-polycytidylic acid(poly Ⅰ:C)and lipopolysaccharides(LPS).AdIFNe1-3 have stronger antiviral activity than AdIFNγ,and AdIFNγ has a stronger antibacterial activity than AdIFNe1-3.The differential responses of these genes to poly Ⅰ:C and LPS suggest differences in the mechanisms of defense against viruses and bacteria.