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摘要We have developed a novel baculovirus surface display (BVSD) system for the isolation of membrane proteins.We expressed a reporter gene that encoded HA fused in frame with the signal peptide and transmembrane domain of the baculovirus gp64 protein which is displayed on the surface of BmNPV virions.The expression of this fusion protein on the virion envelope allowed us to develop two methods for isolating membrane proteins.In the fhrst method,we isolated proteins directly from the envelope of budding BmNPV virions,h the second method,we isolated proteins from cellular membranes that had disintegrated due to viral egress.We isolated a total of 6,756 proteins.Of these,1,883 have sequence similarities to membrane proteins;1,550 proteins are homologous to known membrane proteins.This study indicates that membrane proteins can be effectively isolated using our BVSD system.Using an analogous method,membrane proteins can be isolated from other eukaryotic organisms,including human beings,by employing a host cell-specific budding virus.