换一批
换一批摘要Aim:To explicit the following questions:a.the regulation and possible mechanisms of PP1 in CaMKIIδ alternative splicing; b.the effects of PP1-mediated splicing of CaMKIIδ in cardiac apoptosis.Methods:Mini-CaMKII gene pCI/CaMKIIδE13-E17 and PP1α,PP1β,PP1γ were co-transfected in HEK-293T cell.PP1γ siRNA and ASF over-expression was used to check the roles of PP1 and ASF.ASF,PP1γ,and CaMKIIδ minigene were co-transfected in HEK-293T cells.Adenovirus Ad-PP1γ or Ad-I1PP1 infection or Tautonycetin was used to study how PP1γ regulate the splicing of CaMKIIδ.
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