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摘要Aims: To characterize the alteration metabolites in healthy young men receiving acupuncture at different adjoin acupoints by using NMR-based metabonomic approach.Main Methods: 40 healthy males were recruited in and 1H nuclear magnetic resonance (NMR)-based metabonomic techniques have been used to detect the plasma metabolic profiles.Three adjoin and commonly used acupoints were selected to electro-acupuncture.Venous blood before and after EA treatment was collected and plasma were obtained for NMR detection.Data from NMR spectrometer were then subjected to principal components analysis (PCA) and OSC-PLS (orthogonal signal correction and partial least squares discriminant analysis).Key findings: Clear separation between groups needling at different acupoints could be seen in the score plots.The most distinguished changes in plasma samples by Carr-Purcell-Meiboom-Gill (CPMG) spin-echo pulse sequence including lactate (δ1.32, 1.33, 4.10-4.12), glucose (δ3.40-4.00), phosphocholine (δ3.24), O-acetyl glycoprotein (O-Ac, δ2.12), N-acetyl glycoproteins (N-Ac, δ2.04), glysine (δ3.54), and alanine (δ1.46).In BPP-LED (bipolar pulse pair-longitudinal eddy current delay) experiments, HDL (δ0.86, 1.26), VLDL/LDL (δ0.82, 1.22, 1.3, 1.98), tyrosine (δ3.18) and UFA (δ5.26, 5.34) were larger changed metabolites.Significance: Acupuncture is an important therapeutic method in traditional Chinese medicine (TCM) and has shown to be effective for treatment of various diseases.However, is acupuncture a simple stressing procedure or there are special features This study provides a moderate systemic investigation on the profiles of small metabolites in biofluids before and after acupuncture, and the results would be helpful for further study of the specificity of acupoints and meridian, which is the core theory of acupuncture.