检索历史 清除

摘要Rotaviruses(RVs) is the major cause of the Primary etiological agent leading to non bacterial Infants and young children gastroenteritis.RV genome comprises 11 segments of double-stranded RNA . RV virus particles have 3 layer, a triple-layered capsid without envelope,a diameter of about 70 nm.Rotavirus capsid protein Vp7 possesses specific immuno-reactivities.In this experiment, the VP7 gene was cloned into Bombyx mori baculovirus DNA.Recombinant Bombyx mori baculovirus was obtained and named BmNPV-VP7.Using BmNPV-VP7 infect BmN cells, the 5th instar silkworm larvae and silkworm pupa.SDS-PAGE and Western blotting detected specific band of 35kD in accordance with VP7 protein size, which proved the successful expression of VP7 protein in silkworm.We use ELISA for the determination of the amount of target protein and the relationship with protein expression and infection time (2-7 days after infection).In Bombyx mori cells, the second day began to sicken to express VP7 protein, VP7 protein expression increased with the time, in the 5 days reached maximum, then began to decline; In the silkworm pupa, VP7 protein expression increased with time.VP7 gene was successfully expressed in silkworm baculovirus genetically engineered vaccine for group A rotavirus research provides a new available materials.These data obtained provide a strong basis for further study on the structure and function of the Vp7.