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换一批Proteolytic activation of Bacillus thuringiensis Vip3Aa protein by Spodoptera exigua midgut protease
摘要Methods Proteolysis assay was performed to assess the proteolytic activation of Vip3Aa by Spodoptera exigua midgut proteases(MJ).N-terminal sequencing was used to determine the cleavage site and involved proteases of Vip3Aa processed by MJ.Proteolytic inhibition assay,site-directed mutagenesis assay and bioassay were further conduct.
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