Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid

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作者： Juergens, Hannes ^[1] ; Varela, Javier A. ^[1] ; de Vries, Arthur R. Gorter ^[1] ; Perli, Thomas ^[1] ; Gast, Veronica J. M. ^[1] ; Gyurchev, Nikola Y. ^[2] ; Rajkumar, Arun S. ^[1] ; Mans, Robert ^[1] ; Pronk, Jack T. ^[1] ; Morrissey, John P. ^[2] ; Daran, Jean-Marc G. ^[2]

作者单位： Delft Univ Technol, Dept Biotechnol, Maasweg 9, NL-2629HZ Delft, Netherlands ^[1] Univ Coll Cork, Ctr Synthet Biol & Biotechnol, Environm Res Inst, Sch Microbiol,APC Microbiome Inst, Cork T12 YN60, Ireland ^[2]

关键词 CRISPR/Cas9 ribozymes Kluyveromyces lactis Kluyveromyces marxianus Ogataea polymorpha Hansenula polymorpha

发布时间 2020-02-27

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