Cryo FIB-SEM: Volume imaging of cellular ultrastructure in native frozen specimens

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作者： Schertel, Andreas ^[1] ; Snaidero, Nicolas ^[2] ; Han, Hong-Mei ^[3] ; Ruhwedel, Torben ^[4] ; Laue, Michael ^[5] ; Grabenbauer, Markus ^[6] ; Moebius, Wiebke

作者单位： Advanced Light and Electron Microscopy, Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Nordufer 20, D-13353 Berlin, Germany ^[1] Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), G?ttingen, Germany ^[2] Carl Zeiss Microscopy GmbH, Training, Application and Support Center (TASC), Carl-Zeiss-Stra?e 22, D-73447 Oberkochen, Germany ^[3] Department of Neurogenetics, Electron Microscopy Facility, Max-Planck-Institute of Experimental Medicine, Hermann-Rein-Stra?e 3, D-37075 G?ttingen, Germany ^[4] Cellular Neuroscience, Max Planck Institute of Experimental Medicine, Hermann-Rein-Stra?e 3, D-37075 G?ttingen, Germany ^[5] Department of Systemic Cell Biology, Max-Planck-Institute of Molecular Physiology, Otto-Hahn-Stra?e 11, D-44227 Dortmund, Germany ^[6]

关键词 Cryo FIB-SEM High-pressure freezing Serial block face imaging 3D reconstruction Mouse optic nerve Bacillus subtilis spores

发布时间 2014-02-28

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