A scintillation proximity assay for the Raf/MEK/ERK kinase cascade: high-throughput screening and identification of selective enzyme inhibitors.
第一作者:
O B,McDonald
第一单位:
Division of Biochemistry, GlaxoWellcome Inc., Research Triangle Park, North Carolina, 27709-13398, USA.
作者:
主题词
氨基酸序列(Amino Acid Sequence);动物(Animals);杆状病毒科(Baculoviridae);碱基序列(Base Sequence);DNA引物(DNA Primers);酶抑制剂(Enzyme Inhibitors);大肠杆菌(Escherichia coli);人类(Humans);动力学(Kinetics);MAP激酶激酶1(MAP Kinase Kinase 1);丝裂原活化蛋白激酶1(Mitogen-Activated Protein Kinase 1);丝裂原激活蛋白激酶激酶类(Mitogen-Activated Protein Kinase Kinases);肽类(Peptides);蛋白酪氨酸激酶类(Protein-Tyrosine Kinases);原癌基因蛋白质c-raf(Proto-Oncogene Proteins c-raf);重组融合蛋白质类(Recombinant Fusion Proteins);闪烁计数(Scintillation Counting);信号传导(Signal Transduction);底物特异性(Substrate Specificity)
DOI
10.1006/abio.1998.3030
PMID
10075822
发布时间
2021-12-03
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Analytical biochemistry
318-29页
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