The prepropeptide of vacuolar aminopeptidase I is necessary and sufficient to target the fluorescent reporter protein GFP to the vacuole of yeast by the Ccvt pathway.
第一作者:
E,Martinez
第一单位:
Centro de Biologia Molecular Severo Ochoa, CSIC, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, Madrid 28049, Spain.
作者:
医学主题词
氨基酸取代(Amino Acid Substitution);氨肽酶类(Aminopeptidases);天冬氨酸内肽酶类(Aspartic Acid Endopeptidases);生物转运(Biological Transport);半胱氨酸内肽酶类(Cysteine Endopeptidases);细胞质(Cytoplasm);酶前体(Enzyme Precursors);真菌蛋白质类(Fungal Proteins);基因, 报告(Genes, Reporter);绿色荧光蛋白质类(Green Fluorescent Proteins);发光蛋白质类(Luminescent Proteins);多酶复合物(Multienzyme Complexes);启动区, 遗传(Promoter Regions, Genetic);蛋白酶体内肽酶复合物(Proteasome Endopeptidase Complex);重组融合蛋白质类(Recombinant Fusion Proteins);酿酒酵母菌(Saccharomyces cerevisiae);酿酒酵母蛋白质类(Saccharomyces cerevisiae Proteins);构效关系(Structure-Activity Relationship);空泡(Vacuoles)
DOI
10.1046/j.1365-2958.1999.01446.x
PMID
10411723
发布时间
2019-07-25
- 浏览5
Molecular microbiology
52-62页
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