A Rubredoxin based system for screening of protein expression conditions and on-line monitoring of the purification process.
作者:
主题词
色谱法, 高压液相(Chromatography, High Pressure Liquid);色谱法, 离子交换(Chromatography, Ion Exchange);克隆, 分子(Cloning, Molecular);大肠杆菌(Escherichia coli);黄素氧还蛋白(Flavodoxin);基因表达(Gene Expression);绿色荧光蛋白质类(Green Fluorescent Proteins);人类(Humans);氢离子浓度(Hydrogen-Ion Concentration);发光蛋白质类(Luminescent Proteins);次氮基三乙酸(Nitrilotriacetic Acid);有机金属化合物(Organometallic Compounds);重组融合蛋白质类(Recombinant Fusion Proteins);红色还原蛋白类(Rubredoxins);温度(Temperature)
DOI
10.1016/s1046-5928(02)00704-0
PMID
12699702
发布时间
2019-11-07
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