A novel expression system for genomic DNA loci using a human artificial chromosome vector with transformation-associated recombination cloning.
作者:
主题词
动物(Animals);印迹法, DNA(Blotting, Southern);CHO细胞(CHO Cells);染色体, 人工, 人(Chromosomes, Artificial, Human);克隆, 分子(Cloning, Molecular);仓鼠亚科(Cricetinae);仓鼠属(Cricetulus);DNA引物(DNA Primers);电泳, 凝胶, 脉冲场(Electrophoresis, Gel, Pulsed-Field);基因表达谱(Gene Expression Profiling);基因组, 人(Genome, Human);人类(Humans);次黄嘌呤磷酸核糖转移酶(Hypoxanthine Phosphoribosyltransferase);原位杂交, 荧光(In Situ Hybridization, Fluorescence);聚合酶链反应(Polymerase Chain Reaction);重组, 遗传(Recombination, Genetic);限制性内切酶图谱法(Restriction Mapping);转化, 遗传(Transformation, Genetic)
DOI
10.1007/s10038-005-0300-6
PMID
16231070
发布时间
2020-02-25
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Journal of human genetics
592-599页
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