Development of microwave-assisted protein digestion based on trypsin-immobilized magnetic microspheres for highly efficient proteolysis followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis.
第一作者:
Shuang,Lin
第一单位:
Department of Chemistry & Institute of Biomedical Sciences, Fudan University, Shanghai 200433, China.
作者:
主题词
细胞色素c类(Cytochromes c);酶类, 固相(Enzymes, Immobilized);磁力学(Magnetics);微球体(Microspheres);微波(Microwaves);肽水解酶类(Peptide Hydrolases);肽谱(Peptide Mapping);光谱法, 质量, 电喷雾电离(Spectrometry, Mass, Electrospray Ionization);光谱法, 质量, 基质辅助激光解吸电离(Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization);胰蛋白酶(Trypsin)
DOI
10.1002/rcm.3283
PMID
17990248
发布时间
2007-11-16
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