Production and characterization of pharmacologically active recombinant human phosphodiesterase 4B in Dictyostelium discoideum.
作者:
主题词
动物(Animals);色谱法, 亲和(Chromatography, Affinity);克隆, 分子(Cloning, Molecular);环核苷酸磷酸二酯酶类, 4型(Cyclic Nucleotide Phosphodiesterases, Type 4);网柱菌属(Dictyostelium);酶抑制剂(Enzyme Inhibitors);遗传载体(Genetic Vectors);人类(Humans);动力学(Kinetics);磷酸二酯酶4抑制剂(Phosphodiesterase 4 Inhibitors);质粒(Plasmids);重组蛋白质类(Recombinant Proteins);底物特异性(Substrate Specificity);转化, 遗传(Transformation, Genetic)
DOI
10.1002/biot.200700256
PMID
18348140
发布时间
2010-11-18
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Biotechnology journal
938-47页
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