Soluble expression in Escherichia coli of active human cyclic nucleotide phosphodiesterase isoform 4B2 in fusion with maltose-binding protein.
第一作者:
Sha,Zhu
第一单位:
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University, Chongqing 400016, China.
作者:
医学主题词
动物(Animals);载体蛋白质类(Carrier Proteins);细胞提取物(Cell Extracts);细胞系, 肿瘤(Cell Line, Tumor);环核苷酸磷酸二酯酶类, 4型(Cyclic Nucleotide Phosphodiesterases, Type 4);大肠杆菌(Escherichia coli);基因表达(Gene Expression);人类(Humans);同工酶类(Isoenzymes);异丙硫半乳糖苷(Isopropyl Thiogalactoside);麦芽糖结合蛋白质类(Maltose-Binding Proteins);重组融合蛋白质类(Recombinant Fusion Proteins);溶解度(Solubility)
DOI
10.1271/bbb.90131
PMID
19352009
发布时间
2010-11-18
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