Preparation of selective and segmentally labeled single-stranded DNA for NMR by self-primed PCR and asymmetrical endonuclease double digestion.
第一作者:
Frank H T,Nelissen
第一单位:
Department of Biophysical Chemistry, Institute for Molecules and Materials, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, the Netherlands.
作者:
主题词
DNA连接酶类(DNA Ligases);DNA引物(DNA Primers);DNA限制酶类(DNA Restriction Enzymes);DNA, 单链(DNA, Single-Stranded);脱氧核糖核苷酸类(Deoxyribonucleotides);同位素标记(Isotope Labeling);核磁共振, 生物分子(Nuclear Magnetic Resonance, Biomolecular);聚合酶链反应(Polymerase Chain Reaction)
DOI
10.1093/nar/gkp540
PMID
19553193
发布时间
2021-10-20
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