Expression of GFP under the control of the RNA helicase VASA permits fluorescence-activated cell sorting isolation of human primordial germ cells.
第一作者:
Katarzyna,Tilgner
第一单位:
North East Stem Cell Institute, Newcastle University, International Centre for Life, NE1 3BZ, United Kingdom.
作者:
医学主题词
细胞分化(Cell Differentiation);细胞系(Cell Line);细胞分离(Cell Separation);染色质组装和分解(Chromatin Assembly and Disassembly);DEAD-box RNA解旋酶类(DEAD-box RNA Helicases);胚胎干细胞(Embryonic Stem Cells);女(雌)性(Female);流式细胞术(Flow Cytometry);基因表达谱(Gene Expression Profiling);基因表达调控, 发育期(Gene Expression Regulation, Developmental);生殖细胞(Germ Cells);绿色荧光蛋白质类(Green Fluorescent Proteins);人类(Humans);减数分裂(Meiosis);显微镜检查, 共焦(Microscopy, Confocal);寡核苷酸序列分析(Oligonucleotide Array Sequence Analysis);启动区, 遗传(Promoter Regions, Genetic);重组蛋白质类(Recombinant Proteins);逆转录聚合酶链反应(Reverse Transcriptase Polymerase Chain Reaction);时间因素(Time Factors);转染(Transfection)
DOI
10.1002/stem.263
PMID
19937754
发布时间
2025-05-29
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