A simple and efficient method for the production of human glycosylated glial cell line-derived neurotrophic factor using a Semliki Forest virus expression system.
第一作者:
Eduardo,Ansorena
第一单位:
Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Navarra, C/Irunlarrea 1, 31008 Pamplona, Spain.
作者:
主题词
动物(Animals);细胞系(Cell Line);仓鼠亚科(Cricetinae);成纤维细胞(Fibroblasts);遗传载体(Genetic Vectors);胶质细胞源性神经营养因子(Glial Cell Line-Derived Neurotrophic Factor);糖基化(Glycosylation);人类(Humans);PC12细胞(PC12 Cells);大鼠(Rats);重组蛋白质类(Recombinant Proteins);西门利克森林病毒(Semliki forest virus)
DOI
10.1016/j.ijpharm.2012.04.071
PMID
22580212
发布时间
2012-12-24
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