Cryopreservation of green fluorescent protein (GFP)-labeled primordial germ cells with GFP fused to the 3' untranslated region of the nanos gene by vitrification of Japanese eel (Anguilla japonica) somite stage embryos.
第一作者:
Y,Kawakami
第一单位:
Nanae Fresh Water Laboratory, Field Science Center of Northern Biosphere, Hokkaido University, Nanae 041-1105, Japan. yutakakjp@hokudai-nanae.jp
作者:
医学主题词
氨基酸序列(Amino Acid Sequence);鳗鲡属(Anguilla);动物(Animals);碱基序列(Base Sequence);克隆, 分子(Cloning, Molecular);低温保存(Cryopreservation);胚胎, 非哺乳动物(Embryo, Nonmammalian);鱼蛋白质类(Fish Proteins);基因表达调控, 发育期(Gene Expression Regulation, Developmental);生殖细胞(Germ Cells);绿色荧光蛋白质类(Green Fluorescent Proteins);分子序列数据(Molecular Sequence Data);种系发生(Phylogeny);RNA, 信使(RNA, Messenger);染色与标记(Staining and Labeling);斑马鱼(Zebrafish)
DOI
10.2527/jas.2011-4884
PMID
22829617
发布时间
2012-12-20
- 浏览26
Journal of animal science
4256-65页
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