换一批
换一批G protein-coupled receptor quantification using peptide group-specific enrichment combined with internal peptide standard reporter calibration.
作者：
关键词
5-HT5-hydroxytryptamine (serotonin)ESIG protein-coupled receptorG-protein coupled receptorGPCRIPAImmunoaffinity-MSLC–MSLLODLLOQMALDIMFIMembrane proteinPERSDRTS/NTXPTriple X proteomicselectrospray ionizationiTRAQ?isobaric tag for relative and absolute quantificationisopropyl alcoholliquid chromatography–mass spectrometrylower limit of detectionlower limit of quantificationmAChRmatrix assisted laser desorption ionizationmean fluorescence intensitymuscarinic acetylcholine receptorphycoerythrinrelative standard deviationretention timesignal-to-noise
主题词
动物(Animals);印迹法, 蛋白质(Blotting, Western);校准(Calibration);酶联免疫吸附测定(Enzyme-Linked Immunosorbent Assay);女(雌)性(Female);肽类(Peptides);蛋白质结构, 三级(Protein Structure, Tertiary);蛋白质组学(Proteomics);大鼠(Rats);大鼠, Sprague-Dawley(Rats, Sprague-Dawley);受体, G-蛋白偶联(Receptors, G-Protein-Coupled);参考标准(Reference Standards)
DOI
10.1016/j.jprot.2013.02.017
PMID
23500134
发布时间
2016-05-18
- 浏览27
Journal of proteomics
85-95页
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