换一批
换一批Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artificial membrane environments.
作者：
关键词
1,2-dimyristoyl-sn-glycero-3-phospho-(1′-rac-glycerol)1,2-dimyristoyl-sn-glycero-3-phosphocholine1,2-dioleoyl-sn-glycero-3-phosphocholine1-myristoyl-2-hydroxy-sn-glycero-3-[phospho-rac-(1-glycerol)]1-palmitoyl-2-hydroxy-sn-glycero-3-[phospho-rac-(1-glycerol)]1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholineAso-PCBTECECFCFCHSCVsCell-free expressionDMPCDMPGDOPCEPLETAETBEscherichia coli polar lipidsFMG-protein coupled receptorGPCRHTLHuman endothelin systemIMACLMPGLPPGLT-SDS-PAGELipid screeningMSPNDNanodiscPBSPOPCPRRMSDS-PAGESPRVasoactive peptidebrain total extractcell-freecholesteryl hemisuccinatecolumn volumescontinuous exchange cell-freeendothelin A receptorendothelin B receptorfeeding mixheart total lipidsimmobilized metal affinity chromatographylow temperature SDS-PAGEmembrane scaffold proteinnanodiscphosphate buffered salineproteorhodopsinreaction mixred shifted green fluorescent proteinsGFPsodium dodecyl sulfate polyacrylamide gel electrophoresessoybean l-α-phosphatidylcholinesurface plasmon resonance
主题词
无细胞系统(Cell-Free System);洗涤剂(Detergents);内皮缩血管肽1(Endothelin-1);基因表达(Gene Expression);人类(Humans);动力学(Kinetics);脂质体(Liposomes);纳米结构(Nanostructures);蛋白质结合(Protein Binding);蛋白质折叠(Protein Folding);受体, 内皮素A(Receptor, Endothelin A);受体, 内皮素B(Receptor, Endothelin B)
DOI
10.1016/j.bbamem.2013.05.031
PMID
23747296
发布时间
2016-11-26
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