换一批
换一批PTHrP in differentiating human mesenchymal stem cells: transcript isoform expression, promoter methylation, and protein accumulation.
作者：
关键词
3,3′,5,5′-tetramethylbenzidine5-bromo-4-chloro-3′-indolyphosphateAdipogenesisBCIPBMPD-PBSDulbecco's phosphate buffered salineEDTAFCSGene expressionMICEMSCMSREMesenchymal stem cellsNBTOsteogenesisPCRPMSFPTHrPPromoter methylationSDSSMTBS-TTMBTris-buffered saline-Tween 20bone morphogenetic proteindNTPsdeoxynucleotide triphosphatesethylenediaminetetraacetic acidfetal calf serummesenchymal stem cellsmethylation-sensitive restriction endonucleasemicrotiter immunocytochemical ELISA assaynitro-blue tetrazoliumparathyroid hormone-related proteinphenylmethylsulfonyl fluoridepolymerase chain reactions.e.m.semi-quantitative multiplexsodium dodecyl sulfatestandard error of the mean
主题词
脂细胞(Adipocytes);选择性剪接(Alternative Splicing);细胞分化(Cell Differentiation);细胞, 培养的(Cells, Cultured);CpG岛(CpG Islands);DNA甲基化(DNA Methylation);外显子(Exons);基因表达调控(Gene Expression Regulation);人类(Humans);内含子(Introns);间质干细胞(Mesenchymal Stem Cells);骨细胞(Osteocytes);甲状旁腺激素相关蛋白质(Parathyroid Hormone-Related Protein);启动区, 遗传(Promoter Regions, Genetic);蛋白质亚型(Protein Isoforms);转录, 遗传(Transcription, Genetic)
DOI
10.1016/j.biochi.2013.06.014
PMID
23810909
发布时间
2018-12-02
- 浏览70
Biochimie
1888-96页
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