换一批A thermoactive secreted phospholipase A₂ purified from the venom glands of Scorpio maurus: relation between the kinetic properties and the hemolytic activity.
第一作者：
Hanen,Louati
第一单位：
Laboratory of Biochemistry and Enzymatic Engineering of Lipases, ENIS Route of Soukra, P.O. Box 1173-3038, University of Sfax, Tunisia.
作者：
关键词
1,2-dilauroyl-sn-Glycero-3-phosphocholine1,2-dilauroyl-sn-Glycero-3-phosphoethanolamineBSAEDTAEGTAGCHPLCHemolytic activityN,N,N′,N′-tetramethyl-ethylenediamineNaTDCPGPLPSPhospholipase A(2)Phospholipids monolayerSDS-PAGEScorpio maurusScorpio maurus phospholipase from venom glandsSm-PLVGTEMEDTLCVenom glandbovine serum albumindiC(12)-PCdiC(12)-PEegg-PCethylenediaminetetraacetic acidethyleneglycoltetraacetic acidgas chromatographyhigh pressure liquid chromatographykDakilodaltonl-α-phosphatidyl-(DL)-glyceroll-α-phosphatidyl-(L)-serinephosphatidylcholine from egg yolkphospholipasesodium dodecyl sulfate polyacrylamide gel electrophoresissodium taurodeoxycholatethin layer chromatography
医学主题词
动物(Animals);化学分馏法(Chemical Fractionation);色谱法, 高压液相(Chromatography, High Pressure Liquid);红细胞(Erythrocytes);溶血剂(Hemolytic Agents);人类(Humans);氢离子浓度(Hydrogen-Ion Concentration);动力学(Kinetics);磷脂酶类A2(Phospholipases A2);兔(Rabbits);大鼠(Rats);蝎毒液类(Scorpion Venoms);蝎(Scorpions)
DOI
10.1016/j.toxicon.2013.06.017
PMID
23831286
发布时间
2013-08-05
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