Complete subsite mapping of a "loopful" GH19 chitinase from rye seeds based on its crystal structure.
作者:
关键词
(GlcNAc)(n)2-acetamido-2-deoxy-β-d-glucopyranose2×(GlcNAc)(4)Chitin oligosaccharideCrystal structureDomain motionGH19 chitinaseGlcNAcMDRSC-cRSC-c-E67Q/W72ARye seeda double mutant RSC-c, in which Glu67 and Trp72 are mutated to glutamine and alanine, respectivelymolecular dynamicstwo molecules of (GlcNAc)(4)wild type GH19 chitinase from rye seedsβ-1,4-linked oligosaccharide of GlcNAc with a polymerization degree of n
主题词
乙酰葡萄糖胺(Acetylglucosamine);催化域(Catalytic Domain);计算机模拟(Computer Simulation);结晶学, X线(Crystallography, X-Ray);配体(Ligands);模型, 分子(Models, Molecular);突变(Mutation);寡糖类(Oligosaccharides);植物蛋白质类(Plant Proteins);蛋白质结合(Protein Binding);种子(Seeds)
DOI
10.1016/j.febslet.2013.07.008
PMID
23871710
发布时间
2016-11-25
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FEBS letters
2691-7页
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