换一批Quantitative capillary zone electrophoresis method for the precise determination of charge differences arising from the manufacture of heparan-N-sulfatase.
第一作者：
Daniel S,Roseman
第一单位：
Shire, Lexington, MA, USA. droseman@shire.com
作者：
关键词
4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acidBGECIEFCITPCZECapillary zone electrophoresisCharge variantEDTAEOFGlycosylationGly–GlyHEPESHNSHPAE-PADHeparan-N-sulfataseHeterogeneityIsoformM6PNP40RSDSDSTergitol-type NP40 nonyl phenoxypolyethoxylethanolTris(hydroxymethyl)aminomethaneTris(hydroxymethyl)aminomethane hydrochlorideTris–HClTris–basebackground electrolyte buffercapillary isoelectric focusingcapillary isotachophoresiscapillary zone electrophoresisdiglycineelectroosmotic flowethylenediaminetetraacetic acidheparan-N-sulfatasehigh-performance anion-exchange pulsed amperometric detectionmannose-6-phosphaterelative standard deviationsodium dodecyl sulphatetergitol-type NP-40 nonyl phenoxypolyethoxylethanol
DOI
10.1016/j.jpba.2013.05.018
PMID
23917036
发布时间
2013-09-09
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