第一作者:
Chunli,Zhang
第一单位:
Max Planck Institute of Biophysics, Department of Molecular Membrane Biology, Max-von-Laue-Str. 3, D-60438 Frankfurt am Main, Germany.
作者:
关键词
AAF(1)F(O)Artificial operonBN-PAGEDDMDMEAF(1)F(O)EMHeterologous expressionHyperthermophilic organismIMACIPTGLC–ESI–MSMALDI-TOF-MSMembrane protein complexNi-NTANi–Nitrilotriacetic acidPMFProtein assemblyRespiratory enzymeSECblue native polyacrylamide gel electrophoresiselectron microscopyimmobilized-metal affinity chromatographyisopropyl-β-thiogalactopyranosideliquid chromatography–electrospray ionization tandem mass spectrometrymatrix-assisted laser desorption-ionization time-of-flight mass spectrometryn-decyl-β-d-maltosiden-dodecyl-β-d-maltosidepeptide mass fingerprintsize-exclusion chromatographytrans-4-(trans-4′-propylcyclohexyl)cyclohexyl-α-d-maltosideΑ. aeolicus ATP synthase heterologously produced in E. coliΑ. aeolicus ATP synthase isolated from native cells of A. aeolicusα-PCC
医学主题词
腺苷三磷酸(Adenosine Triphosphate);动物(Animals);印迹法, 蛋白质(Blotting, Western);催化作用(Catalysis);色谱法, 凝胶(Chromatography, Gel);大肠杆菌(Escherichia coli);革兰氏阴性菌(Gram-Negative Bacteria);水解作用(Hydrolysis);免疫球蛋白G(Immunoglobulin G);线粒体质子转运ATP酶(Mitochondrial Proton-Translocating ATPases);肽碎片(Peptide Fragments);兔(Rabbits);重组蛋白质类(Recombinant Proteins);光谱法, 质量, 基质辅助激光解吸电离(Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization)
DOI
10.1016/j.bbagen.2013.08.023
PMID
24005236
发布时间
2016-11-26
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