换一批
第一作者：
Kang Lan,Tee
第一单位：
Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, England, United Kingdom.
作者：
关键词
2′-Deoxy-P-nucleoside-5′-triphosphate2′-Deoxyinosine 5′-triphosphate7-Deaza-7-(triethylsilylethynyl)deoxyadenosine triphosphate8-Oxo-2′-deoxyguanosine-5′-triphosphate8-oxo-dGTPAAVABI-RECAdeno-associated virusAsymmetric Bridge PCR with Intramolecular Homologous RecombinationBase pairsCASTCPECCRPCircular Polymerase Extension CloningCombinatorial Active-Site Saturation TestDGRsDNADNA recombinationDeoxyribonucleic acidDeoxyribonucleotide triphosphateDirected evolutionDiversity-generating retroelementsDouble-stranded DNADuARCheMDual Approach to Random Chemical MutagenesisEMPEMSEnoyl-acyl carrier protein reductaseError-prone polymerase chain reactionError-prone rolling circle amplificationEthyl methane sulfonateExponential Megapriming PCRGOIGSTGene of interestGenetic diversityGenome engineeringGlutathione-S-transferaseITCHYInDelIncremental Truncation for the Creation of Hybrid EnzymesInsertion and deletionKFKlenow fragmentMAPMEGAWHOPMGSMLF-SDMMegaprimed and Ligase-Free PCR-based Method for Site-Directed MutagenesisMegaprimer PCR of Whole PlasmidMetabolic engineeringMutagenesis Assistant ProgramMutation Generation SystemNEBNRRNew England BiolabsNiDENicking DNA EndonucleaseNon-homologous random recombinationNucleotideOLTAOSCARROne-pot Simple Methodology for Casette Randomization and RecombinationOverLap extension PCR and TA cloningPCRPCR Production of Circular PlasmidPERMUTEPERMutation Using Transposase EngineeringPFLF-MSDMPLICingPPCPPSPTRecPhosphorothioatePhosphorothioate-based DNA RecombinationPhosphorothioate-based Ligase-Independent Gene CloningPhosphorylation-Free and Ligase-Free PCR-based Method for Multiple SDMPolymerase chain reactionRCAREsRF cloningRGENRNA-guided EndonucleaseRandom mutagenesisRestriction enzymesRestriction-Free cloningRolling circle amplificationSDMSEFCSHIPRECSLiCESPRINPSTRU-CloningSeSaMSeamless Enzyme-Free CloningSeamless Ligation Cloning ExtractSequence Homology-Independent Protein RECombinationSequence Saturation MutagenesisSingle-Primer Reactions In ParallelSingle-Tube Restriction-based Ultrafiltration CloningSingle-stranded DNASite-directed mutagenesisStEPStaggered Extension ProcessSynthetic biologyT(s)T(v)TALENsTAMTIMTMGS-PCRTPCRTRINSTaGTEAMTandem Repeat InsertionTargeting Glycosylases To Embedded Arrays for MutagenesisTranscription activator-like effector nucleasesTranscription-associated mutationTransfer-PCRTransitionsTransposon Integration mediated MutagenesisTransversionsTriNExTriNucleotide EXchangeTruncated Metagenomic Gene-Specific PCRUDGUSER Friendly DNA RecombinationUSERecUracil-DNA glycosylaseZFNZinc finger nucleasebpcAMP receptor proteindA(TESE)TPdITPdNTPdPTPdsDNAenoyl ACP reductaseepPCRepRCAntp-BenzoylphenylalaninepBpassDNA
医学主题词
动物(Animals);生物工程(Bioengineering);克隆, 分子(Cloning, Molecular);定向分子进化(Directed Molecular Evolution);基因文库(Gene Library);遗传变异(Genetic Variation);人类(Humans);小鼠(Mice);诱变, 定点(Mutagenesis, Site-Directed)
DOI
10.1016/j.biotechadv.2013.08.021
PMID
24012599
发布时间
2022-04-08
- 浏览118
Biotechnology advances
1707-21页
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