The multiple forms of bovine seminal ribonuclease: structure and stability of a C-terminal swapped dimer.
作者:
关键词
BS-RNaseBovine seminal ribonucleaseC-terminal 3D domain swappingCytotoxic RNaseD(ART)-BSD(ART)-STAA-BSDTTG16S/N17T/P19A/S20A variant of BS-RNaseG16S/N17T/P19A/S20A variant of mBSHinge peptideIAAL28Q variant of mBSL28Q-mBSM=MMultiple swappingM×MNCD-BSP19A/L28Q variant of BS-RNAseP19A/L28Q variant of mBSPALQ-BS-RNasePALQ-mBSR80S variant of STAA-BS-RNaseR80S variant of STAA-mBSR80S-STAA-BS-RNaseR80S-STAA-mBSRIRNase ASECSTAA-BS-RNaseSTAA-mBSartificial dimer of BS-RNase obtained by incubation in acetic acid of mBS and lyophilisationartificial dimer of STAA-BS-RNase obtained by incubation in acetic acid of STAA-mBS and lyophilisationbovine pancreatic ribonuclease (EC 3.1.2.27.5)bovine seminal ribonucleasecovalent swapped isomer of BS-RNasecovalent unswapped isomer of BS-RNasedithiothreitoliodoacetamidemBSmonomeric derivative of BS-RNase with cysteines 31 and 32 either linked to glutathione moieties or alkylated with iodoacetamidenon-covalent swapped dimer of BS-RNase with cysteines 31 and 32 linked to iodoacetamide, obtained by selective reduction of the swapped isoform and iodoalkylationribonuclease inhibitorsize-exclusion chromatography
主题词
氨基酸序列(Amino Acid Sequence);动物(Animals);牛(Cattle);结晶学, X线(Crystallography, X-Ray);内切核糖核酸酶类(Endoribonucleases);分子序列数据(Molecular Sequence Data);突变(Mutation);蛋白质多聚化(Protein Multimerization);蛋白质结构, 四级(Protein Structure, Quaternary);蛋白质结构, 三级(Protein Structure, Tertiary)
DOI
10.1016/j.febslet.2013.10.003
PMID
24140346
发布时间
2013-11-19
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FEBS letters
3755-62页
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