换一批Mutation of a pH-modulating residue in a GH51 α-l-arabinofuranosidase leads to a severe reduction of the secondary hydrolysis of transfuranosylation products.
第一作者：
Bastien,Bissaro
第一单位：
Université de Toulouse; INSA, UPS, INP; LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; INRA, UMR792, Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France.
作者：
关键词
4-nitrochatecol α-l-arabinofuranoside4NTC-α-l-ArafAXOSAbfsFHGHKIEPentoses/furanosesR(T)STD NMRSaturation Transfer Difference Nuclear Magnetic ResonanceT/H ratioTransglycosylationTxAbfTxAbf(?)XYarabinoxylo-oligosaccharidesd-Xylpd-xylopyranosyldonor conversion ratefuranoside hydrolaseglycoside hydrolaseinactivated form (E176A) of TxAbfkinetic isotope effectsl-Arafl-arabinofuranosylmNPmeta-nitrophenoloNPortho-nitrophenolpH-dependent inhibitionpK(a) modulationpNPpNP-α-l-Arafpara-nitrophenolpara-nitrophenyl α-l-arabinofuranosidetransfer ratetransglycosylation/hydrolysis ratioyieldα-l-arabinofuranosidase from Thermobacillus xylanilyticusα-l-arabinofuranosidases
医学主题词
阿拉伯糖(Arabinose);芽孢杆菌科(Bacillaceae);催化作用(Catalysis);催化域(Catalytic Domain);色谱法, 薄层(Chromatography, Thin Layer);糖苷水解酶类(Glycoside Hydrolases);糖基化(Glycosylation);氢离子浓度(Hydrogen-Ion Concentration);水解作用(Hydrolysis);动力学(Kinetics);磁共振波谱学(Magnetic Resonance Spectroscopy);模型, 分子(Models, Molecular);诱变, 定点(Mutagenesis, Site-Directed);突变(Mutation);底物特异性(Substrate Specificity)
DOI
10.1016/j.bbagen.2013.10.013
PMID
24140392
发布时间
2016-11-26
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