Identification and expression pattern analysis of Piwi genes during the spermiogenesis of Portunus trituberculatus.
第一作者:
Dong-Fang,Xiang
第一单位:
The Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China; School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, China.
作者:
关键词
3-Aminopropyl-triethoxysilane5-bromo-4-chloro-3-indolylphosphateAACAPESATAgo3Argonaute3BCIPDEPCDIGDigoxigeninEVGSPILMCMLNBTNJNUPNested Universal PrimerOLORFP-element induced wimpy testisPAZPBSPFAPGPMPVPiwiPiwi–Argonaute–ZwillePortunus trituberculatusRARACERT-PCRSSCSpermiogenesisTD-PCRTestisUPMUTRUniversal Primer Mixaaacrosomal tubuleacrosomeamino acidsapical capdiethylpyrocarbonateendoplasmic reticulum vesiclegene specific primerinner layermembrane complexmiddle layerneighbor-joiningnitroblue tetrazolium chlorideopen reading frameouter layerparaformaldehydephosphate-buffered salinepiRNApiwi-interacting RNAplasma membraneproacrosomal granuleproacrosomal vesicleradial armsrapid-amplification of cDNA endsreverse transcription-polymerase chain reactionstandard saline citratetouchdown PCRuntranslated region
主题词
氨基酸序列(Amino Acid Sequence);动物(Animals);碱基序列(Base Sequence);短尾派(Brachyura);克隆, 分子(Cloning, Molecular);DNA, 互补(DNA, Complementary);分子序列数据(Molecular Sequence Data);种系发生(Phylogeny);蛋白质结构, 三级(Protein Structure, Tertiary);序列比对(Sequence Alignment);序列分析, DNA(Sequence Analysis, DNA);精子发生(Spermatogenesis)
DOI
10.1016/j.gene.2013.10.050
PMID
24211384
发布时间
2013-12-20
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Gene
240-8页
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