Standardization of α-L-iduronidase enzyme assay with Michaelis-Menten kinetics.

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第一作者： Li,Ou

第一单位： Department of Genetics, Cell Biology and Development, PWB 13-146, 516 Delaware Str SE, Minneapolis, MN 55455, USA; Molecular, Cellular, Developmental Biology & Genetics Graduate Program, PWB 13-146, 516 Delaware Str SE, Minneapolis, MN 55455, USA; Gene Therapy Center, Department of Pediatrics, University of Minnesota, USA.

作者： Li,Ou ^[1] ; Tyler L,Herzog ^[2] ; Carrie M,Wilmot ^[3] ; Chester B,Whitley ^[4]

作者单位： Department of Genetics, Cell Biology and Development, PWB 13-146, 516 Delaware Str SE, Minneapolis, MN 55455, USA; Molecular, Cellular, Developmental Biology & Genetics Graduate Program, PWB 13-146, 516 Delaware Str SE, Minneapolis, MN 55455, USA; Gene Therapy Center, Department of Pediatrics, University of Minnesota, USA. ^[1] Department of Biochemistry, Molecular Biology and Biophysics, 5-120 NHH, 312 Church Str SE, Minneapolis, MN 55455, USA; Gene Therapy Center, Department of Pediatrics, University of Minnesota, USA. ^[2] Department of Biochemistry, Molecular Biology and Biophysics, 5-120 NHH, 312 Church Str SE, Minneapolis, MN 55455, USA. ^[3] Molecular, Cellular, Developmental Biology & Genetics Graduate Program, PWB 13-146, 516 Delaware Str SE, Minneapolis, MN 55455, USA; Gene Therapy Center, Department of Pediatrics, University of Minnesota, USA. Electronic address: whitley@umn.edu. ^[4]

关键词 4-methylumbelliferyl Hurler syndrome IDUA Lineweaver–Burk plot MPS MU Michaelis–Menten kinetics mucopolysaccharidoses mucopolysaccharidosis α-L-iduronidase α-L-iduronidase enzyme assay