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Expression, purification, and projection structure by single particle electron microscopy of functional human TRPM4 heterologously expressed in Xenopus laevis oocytes.

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第一作者： Benjamin,Clémençon

第一单位： Institute of Biochemistry and Molecular Medicine (IBMM), NCCR TransCure, University of Bern, Bern, Switzerland. Electronic address: benjamin.clemencon@ibmm.unibe.ch.

作者： Benjamin,Clémençon ^[1] ; Michael,Fine ^[2] ; Benjamin,Lüscher ^[3] ; Marc U,Baumann ^[3] ; Daniel V,Surbek ^[3] ; Hugues,Abriel ^[4] ; Matthias A,Hediger ^[2]

作者单位： Institute of Biochemistry and Molecular Medicine (IBMM), NCCR TransCure, University of Bern, Bern, Switzerland. Electronic address: benjamin.clemencon@ibmm.unibe.ch. ^[1] Institute of Biochemistry and Molecular Medicine (IBMM), NCCR TransCure, University of Bern, Bern, Switzerland. ^[2] Department of Obstetrics and Gynecology, University Hospital of Bern, Bern, Switzerland; Department of Clinical Research, University of Bern, Bern, Switzerland. ^[3] Department of Clinical Research, NCCR TransCure, University of Bern, Bern, Switzerland. ^[4]

关键词 Hi clamp two-electrode voltage clamp (TEV) system Human TRPM4 Membrane protein purification Roboinject system Single particle analysis (SPA)Transmission electron microscopy (TEM)Xenopus laevis oocytes

医学主题词动物(Animals);显微镜检查, 电子, 透射(Microscopy, Electron, Transmission);卵母细胞(Oocytes);重组蛋白质类(Recombinant Proteins);TRPM阳离子通道(TRPM Cation Channels);光滑爪蟾(Xenopus laevis)

DOI 10.1016/j.pep.2013.11.017

PMID 24333049

发布时间 2014-02-18

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