The interrelationship between ligand binding and thermal unfolding of the folate binding protein. The role of self-association and pH.

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第一作者： Jan,Holm

第一单位： Department of Clinical Biochemistry, Nordsjællands Hospital-Hillerød, University Hospital Copenhagen, Dyrehavevej 29, DK-3400 Hillerød, Denmark. Electronic address: Janf@holm.mail.dk.

作者： Jan,Holm ^[1] ; Linnea N,Babol ^[2] ; Natalia,Markova ^[2] ; Anders J,Lawaetz ^[3] ; Steen I,Hansen ^[4]

作者单位： Department of Clinical Biochemistry, Nordsjællands Hospital-Hillerød, University Hospital Copenhagen, Dyrehavevej 29, DK-3400 Hillerød, Denmark. Electronic address: Janf@holm.mail.dk. ^[1] GE Healthcare, Life Sciences Bio-Sciences AB, Bjørkgatan 30, 751 84 Uppsala, Sweden. ^[2] Faculty of Science, Department of Food Science, Spectroscopy and Chemometrics, University of Copenhagen, Rolighedsvej 26, DK-1958 Frederiksberg, Denmark. ^[3] Department of Clinical Biochemistry, Nordsjællands Hospital-Hillerød, University Hospital Copenhagen, Dyrehavevej 29, DK-3400 Hillerød, Denmark. Electronic address: Steen.Ingemann.Hansen@Regionh.dk. ^[4]

关键词 Differential scanning calorimetry Dynamic light scattering Fluorescence changes associated with irreversible thermal unfolding Folate binding protein (soluble folate receptor)Temperature-dependent self-association Thermoresistant holo-protein