Purification of supercoiled plasmid DNA from clarified bacterial lysate by arginine-affinity chromatography: effects of spacer arms and ligand density.

导出 LinkOut

翻译打印收藏纠错

第一作者： Jin-Shan,Bai

第一单位： Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.

作者： Jin-Shan,Bai ^[1] ; Shu,Bai ; Qing-Hong,Shi ; Yan,Sun

作者单位： Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China. ^[1]

关键词 Arginine chromatography Dynamic binding capacity High-density ligands Plasmid purification Supercoiled plasmids

主题词精氨酸(Arginine);色谱法, 亲和(Chromatography, Affinity);DNA, 细菌(DNA, Bacterial);DNA, 超螺旋(DNA, Superhelical);大肠杆菌(Escherichia coli);配体(Ligands);质粒(Plasmids)

DOI 10.1002/jssc.201400092

PMID 24723568

发布时间 2019-12-10

翻译满意度评价：

准确一般字义偏差语意偏离译文作用小提交