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首页> Journal of virological methods> Optimization of the elution buffer and concentration method for detecting hepatitis E virus in swine liver using a nested reverse transcription-polymerase chain reaction and real-time reverse transcription-polymerase chain reaction.

Optimization of the elution buffer and concentration method for detecting hepatitis E virus in swine liver using a nested reverse transcription-polymerase chain reaction and real-time reverse transcription-polymerase chain reaction.

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作者： Na Ry,Son ^[1] ; Dong Joo,Seo ^[1] ; Min Hwa,Lee ^[1] ; Sheungwoo,Seo ^[1] ; Xiaoyu,Wang ^[1] ; Bog-Hieu,Lee ^[2] ; Jeong-Su,Lee ^[3] ; In-Sun,Joo ^[3] ; In-Gyun,Hwang ^[3] ; Changsun,Choi ^[4]

作者单位： Department of Food and Nutrition, Chung-Ang University, Republic of Korea. ^[1] Department of Food and Nutrition, Chung-Ang University, Republic of Korea; School of Food Science and Technology, Chung-Ang University, Republic of Korea. ^[2] Food Microbiology Division, Ministry of Food and Drug Safety, Republic of Korea. ^[3] Department of Food and Nutrition, Chung-Ang University, Republic of Korea; School of Food Science and Technology, Chung-Ang University, Republic of Korea. Electronic address: cchoi@cau.ac.kr. ^[4]

关键词 Concentration Elution Hepatitis E virus (HEV)Nested reverse transcription-polymerase chain reaction (nested RT-PCR)Real-time reverse transcription-polymerase chain reaction (real-time RT-PCR)

主题词动物(Animals);缓冲剂(Buffers);化学沉淀(Chemical Precipitation);过滤(Filtration);戊型肝炎(Hepatitis E);戊型肝炎病毒(Hepatitis E virus);氢离子浓度(Hydrogen-Ion Concentration);肝(Liver);聚合酶链反应(Polymerase Chain Reaction);逆转录聚合酶链反应(Reverse Transcriptase Polymerase Chain Reaction);敏感性与特异性(Sensitivity and Specificity);猪(Swine);猪疾病(Swine Diseases);时间因素(Time Factors)

DOI 10.1016/j.jviromet.2014.05.026

PMID 24907649

发布时间 2019-12-10

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Journal of virological methods

99-104页

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